Dale D. Vandré, Ph.D.

Associate Professor
Department of Physiology and Cell Biology

Degree: Biochemistry, University of Iowa
Postdoctoral Training: University of Wisconsin, Madison, Dr. Gary G. Borisy

PHONE: (614) 292-4181
FAX: (614) 292-4888
E-MAIL: vandre.1@osu.edu

Research Area:

Research activities are directed towards the identification and characterization of microtubule associated protein (MAP) phosphorylation events that play a role in the function of the neuronal cytoskeleton, the differentiation of neuronal cells, and in the pathogenesis of Alzheimer's disease.

Current Research:

The functional properties of MAPs are regulated by alterations in the phosphorylation state of these proteins.  We have recently demonstrated that a phosphoepitope recognized by the MPM-2 monoclonal antibody is localized to pathological structures associated with neurodegenerative processes found in Alzheimer's diseased brain samples.  These phosphoepitopes are conserved with phosphoepitopes that are associated with mitotic progression and the assembly/disassembly of the mitotic spindle. We are generating immunological probes that are specific for the recognition of these phosphorylation sites, and are in the process of biochemically defining the phosphoepitope sequence recognized in MAPs by the MPM-2 antibody.  Future studies will focus on the identification and characterization of the kinases responsible for these phosphorylation events, characterization of proteins that interact with phosphorylated MAPs, as well as further characterization of the mitotic phosphoproteins involved in the regulation of microtubule nucleation by the centrosome in both mitotic and postmitotic differentiated cells.

Collaborative efforts with Dr. John Robinson, are examining the unique properties of the microtubule cytoskeleton found in human leukocytes.  These microtubules have been shown to be extremely dynamic, and this may play an important role in the biological function of these cells.  We are developing immunocytochemical methodologies appropriate for the ultrastructural analysis of cytoskeletal and centrosomal structure in these cells.

This lab is also involved with the Experimental Therapeutics Program associated with the Comprehensive Cancer Center at Ohio State University Hospitals.  We are examining the effects of new antitumor drugs on microtubules in selected cell culture lines and patient samples as a correlation with ongoing clinical trials.  In addition, we are examining preclinical activities of other novel chemotherapeutic agents that target the cytoskeleton or cell-cycle regulatory processes.

Techniques:

• protein purification
• gel electrophoresis
• antibody generation
• immunocytochemistry
• electron microscopy
• kinase assay
• cell culture